B. Kinnex Single Cell - Overview
=================================
.. image:: ../_images/kinnex_sc_pb.png
    :scale: 45%
    :alt: Novel Methods and R&D
    :align: right

The PacBio Kinnex™ single-cell RNA kit takes as input single-cell cDNA and outputs a sequencing-ready library that
results in a 16-fold throughput increase compared to
regular single-cell Iso-Seq® libraries. More details on the sequencing platform can be found here: `PacBio's Single Cell FAQs <https://www.pacb.com/products-and-services/applications/rna-sequencing/single-cell-rna-sequencing/>`_

Complete application note published by Pacbio can be found here:
`Application note <https://www.pacb.com/wp-content/uploads/Application-note-Kinnex-single-cell-RNA-kit-for-single-cell-isoform-sequencing.pdf>`_

Quick notes:

• 10x Chromium Single Cell 3’ kit (v3.1) and 5’ kit (v2)
• 15–75 ng cDNA input
• 3,000 to 10,000 target cell recovery
• 2-day Kinnex library preparation using Kinnex single-cell RNA kit
• Barcoded Kinnex adapters support up to 4-plex multiplexing
• ~80MN reads



Kinnex Single Cell workflow overview:
-------------------------------------
.. figure:: ../_images/kinnex_sc.png
   :alt: Novel Methods and R&D
   :align: left