A. Kinnex Full Length (bulk) - Overview

A. Kinnex Full Length (bulk) - Overview#

Novel Methods and R&D

The PacBio Kinnex™ full-length RNA kit takes total RNA as input and outputs a sequencing-ready library that results in an 8-fold throughput increase compared to typical IsoSeq libraries.

Complete application note published by Pacbio for Kinnex Full Length can be found here : Application Note

Quick Notes:

  • Input 300 ng total RNA, RIN ≥7

  • Generate up to 12-plex barcoded cDNA using (Iso-Seq express 2.0 kit)

  • 2-day Kinnex library preparation using Kinnex full-length RNA kit

  • Barcoded Kinnex adapters support up to 4-plex multiplexing

  • Total 48 samples per flowcell

  • ~40 million cDNA sequences

Here is a schematic representation of the multi-plexing scheme:

../_images/bulk_multiplexing_scheme.png

Overall Workflow in a nutshell#

Novel Methods and R&D