B. Kinnex Single Cell - Overview

B. Kinnex Single Cell - Overview#

Novel Methods and R&D

The PacBio Kinnex™ single-cell RNA kit takes as input single-cell cDNA and outputs a sequencing-ready library that results in a 16-fold throughput increase compared to regular single-cell Iso-Seq® libraries. More details on the sequencing platform can be found here: PacBio’s Single Cell FAQs

Complete application note published by Pacbio can be found here: Application note

Quick notes:

  • 10x Chromium Single Cell 3’ kit (v3.1) and 5’ kit (v2)

  • 15–75 ng cDNA input

  • 3,000 to 10,000 target cell recovery

  • 2-day Kinnex library preparation using Kinnex single-cell RNA kit

  • Barcoded Kinnex adapters support up to 4-plex multiplexing

  • ~80MN reads

Kinnex Single Cell workflow overview:#

Novel Methods and R&D